The amino acid sequence analysis of protein polypeptide chain, that is, the determination of protein primary structure, mainly includes the following steps:At the same time, Chemical Custom synthesis It is no longer a relatively unfamiliar industry, and it enters the public’s sight more and more frequently, gaining more attention and recognition. https://www.all-chemistry.com/
1. separating and purifying protein to obtain a certain amount of pure protein;
2. Take a certain amount of samples for complete hydrolysis, and then determine the amino acid composition of protein;
3. Analyze the N- terminal and C- terminal amino acids of protein;
4. Treating protein into several peptide segments by using specific enzymes (such as chymotrypsin) or chemical reagents (such as hydrogen bromide);
5. separating and purifying a single peptide segment;
The amino acid sequence of each peptide was determined. Edman degradation method is generally used, and phenyl isothiocyanate is used to react, and amino acids are degraded and determined one by one.
7. Treating protein with at least two different methods to obtain the amino acid sequence of its peptide;
8. Compare the amino acid sequences of two different peptide groups to obtain the complete amino acid sequence of protein molecule.